CHRNA10 Polyclonal antibody

CHRNA10 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 55291-1-AP

Synonyms

CHRNA10, NACHR alpha 10, NACHRA10

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Validation Data Gallery

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  WB analysis of Raji using 55291-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55291-1-AP (CHRNA10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55291-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55291-1-AP (CHRNA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55291-1-AP targets CHRNA10 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cholinergic receptor, nicotinic, alpha 10
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-52 kDa
• GenBank Accession Number: NM_020402
• Gene Symbol: CHRNA10
• Gene ID (NCBI): 57053
• RRID: AB_10860270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHRNA10, also named as NACHRA10, belongs to the ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily and Alpha-10/CHRNA10 sub-subfamily. It is an ionotropic receptor with a probable role in the modulation of auditory stimuli. In the ear, CHRNA10 may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. It may protect against acoustic trauma. This antibody is specific to CHRNA10.