CHRNA5 Polyclonal antibody

CHRNA5 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 13516-1-AP

Synonyms

CHRNA5, LNCR2, NACHRA5



Tested Applications

Positive WB detected inJurkat cells, A431 cells, A549 cells, mouse bladder tissue, mouse stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

13516-1-AP targets CHRNA5 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNA5 fusion protein Ag4447
Full Name cholinergic receptor, nicotinic, alpha 5
Calculated molecular weight 468 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC033639
Gene symbol CHRNA5
Gene ID (NCBI) 1138
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

Protocols

Product Specific Protocols
WB protocol for CHRNA5 antibody 13516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols