Tested Applications
| Positive WB detected in | mouse thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11388-1-AP targets CHRNA6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1921 Product name: Recombinant human CHRNA6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-251 aa of BC014456 Sequence: MLTSKGQGFLHGGLCLWLCVFTPFFKGCVGCATEERLFHKLFSHYNQFIRPVENVSDPVTVHFEVAITQLANVDEVNQIMETNLWLRHIWNDYKLRWDPMEYDGIETLRVPADKIWKPDIVLYNNAVGDFQVEGKTKALLKYNGMITWTPPAIFKSSCPMDITFFPFDHQNCSLKFGSWTYDKAEIDLLIIGSKVDMNDFWENSEWEIIDASGYKHDIKYNCCEEIYTDITYSFYIRRLPMFYTINLIIPC Predict reactive species |
| Full Name | cholinergic receptor, nicotinic, alpha 6 |
| Calculated Molecular Weight | 494 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC014456 |
| Gene Symbol | CHRNA6 |
| Gene ID (NCBI) | 8973 |
| RRID | AB_2080683 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15825 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHRNA6 (α6) is an α subunit of neuronal nicotinic acetylcholine receptors (nAChRs), encoded by the CHRNA6 gene on chromosome 8. It assembles into pentameric ligand-gated cation channels permeable to Na⁺ and Ca²⁺, often clustered with CHRNB3. It is restrictively expressed mainly in midbrain dopaminergic neurons, noradrenergic neurons and visual system, modulating nicotine-triggered dopamine release. CHRNA6 polymorphisms link to nicotine and alcohol dependence. It participates in reward circuits and is implicated in Parkinson's disease, serving as a promising target for anti-addiction therapeutics.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHRNA6 antibody 11388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHRNA6 Polyclonal antibody (Cat# 11388-1-AP) has 3 total citations. These appear in Marine Drugs (2 papers) and the European Journal of Pharmacology (1 paper). The associated research fields focus on cancer and neuropharmacology, specifically examining nicotinic acetylcholine receptor expression in human breast cancer, cervical cancer, and lung cancer cell lines, along with the antitumor effects of α-conotoxins. All citations involve human samples, with Western blot as the primary application.
| Species | Application | Title |
|---|---|---|
Mar Drugs Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA. | ||
Eur J Pharmacol Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth. | ||
Mar Drugs Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets. |
