CHST12 Polyclonal antibody

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  WB analysis of K-562 using 15341-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15341-1-AP (CHST12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15341-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15341-1-AP (CHST12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15341-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15341-1-AP (CHST12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15341-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15341-1-AP (CHST12 antibody) at dilution of 1:50 (under 40x lens).

CHST12 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15341-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C4S 2, C4ST 2, C4ST2, CHST12, Sulfotransferase Hlo

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 6 publications below

Product Information

15341-1-AP targets CHST12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7544

  Product name: Recombinant human CHST12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 64-414 aa of BC002918

  Sequence: SDVDEFLDKFLSAGVKQSDLPRKETEQPPAPGSMEESVRGYDWSPRDARRSPDQGRQQAERRSVLRGFCANSSLAFPTKERAFDDIPNSELSHLIVDDRHGAIYCYVPKVACTNWKRVMIVLSGSLLHRGAPYRDPLRIPREHVHNASAHLTFNKFWRRYGKLSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLRLYANHTSLPASAREAFRAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQIDYDFVGKLETLDEDAAQLLQLLQVDRQLRFPPSYRNRTASSWEEDWFAKIPLAWRQQLYKLYEADFVLFGYPKPENLLRD

• Full Name: carbohydrate (chondroitin 4) sulfotransferase 12
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC002918
• Gene Symbol: CHST12
• Gene ID (NCBI): 55501
• RRID: AB_2079046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRB3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Osteoarthritis Cartilage; Altered expression of chondroitin sulfate structure modifying sulfotransferases in the articular cartilage from adult osteoarthritis and Kashin-Beck disease.; Authors - Jing Han
human	IHC	Histochem Cell Biol; Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease.; Authors - Jian Lei
human	WB	Biol Trace Elem Res; Serious Selenium Deficiency in the Serum of Patients with Kashin-Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes.; Authors - Liyun Wang
human	IHC	Biol Trace Elem Res; Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children with Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused by Selenium Deficiency.; Authors - Yijie Guo
rat	IHC	J Trace Elem Med Biol; The effects of long-term low selenium diet on the expression of CHST-3, CHST-12 and UST in knee cartilage of growing rats; Authors - Liyun Wang
rat	WB,IHC	Carbohydr Polym; Nano-elemental selenium particle developed via supramolecular self-assembly of chondroitin sulfate A and Na2SeO3 to repair cartilage lesions; Authors - Jing Han