CHST12 Polyclonal antibody

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• CHST12 Polyclonal antibody (15341-1-AP)

CHST12 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15341-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C4S 2, C4ST 2, C4ST2, Carbohydrate sulfotransferase 12, Chondroitin 4-O-sulfotransferase 2

• 

  WB analysis of K-562 using 15341-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15341-1-AP (CHST12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 15341-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15341-1-AP (CHST12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 15341-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15341-1-AP (CHST12 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 15341-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15341-1-AP (CHST12 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15341-1-AP targets CHST12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7544

  Product name: Recombinant human CHST12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 64-414 aa of BC002918

  Sequence: SDVDEFLDKFLSAGVKQSDLPRKETEQPPAPGSMEESVRGYDWSPRDARRSPDQGRQQAERRSVLRGFCANSSLAFPTKERAFDDIPNSELSHLIVDDRHGAIYCYVPKVACTNWKRVMIVLSGSLLHRGAPYRDPLRIPREHVHNASAHLTFNKFWRRYGKLSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLRLYANHTSLPASAREAFRAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQIDYDFVGKLETLDEDAAQLLQLLQVDRQLRFPPSYRNRTASSWEEDWFAKIPLAWRQQLYKLYEADFVLFGYPKPENLLRD

• Full Name: carbohydrate (chondroitin 4) sulfotransferase 12
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC002918
• Gene Symbol: CHST12
• Gene ID (NCBI): 55501
• RRID: AB_2079046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRB3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-CHST12 antibody (Cat# 15341-1-AP) has 7 total citations. These appear in Carbohydrate Polymers, Osteoarthritis and Cartilage, Bioengineered, Biological Trace Element Research, Journal of Trace Elements in Medicine and Biology, and Histochemistry and Cell Biology. The research fields span cartilage biology, osteoarthritis, Kashin-Beck disease (selenium deficiency), chondroitin sulfate sulfotransferase expression, and glioblastoma cell proliferation via the WNT/β-catenin pathway. Applications include WB and IHC in human and rat samples.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB,IHC	Carbohydr Polym; Nano-elemental selenium particle developed via supramolecular self-assembly of chondroitin sulfate A and Na2SeO3 to repair cartilage lesions; Authors - Jing Han
human	IHC	Osteoarthritis Cartilage; Altered expression of chondroitin sulfate structure modifying sulfotransferases in the articular cartilage from adult osteoarthritis and Kashin-Beck disease.; Authors - Jing Han
human	WB,IHC	Bioengineered; Knockdown of carbohydrate sulfotransferase 12 decreases the proliferation and mobility of glioblastoma cells via the WNT/β-catenin pathway; Authors - Juan Wang ; KD Validated
human	IHC	Biol Trace Elem Res; Decreased Expression of CHST-12, CHST-13, and UST in the Proximal Interphalangeal Joint Cartilage of School-Age Children with Kashin-Beck Disease: an Endemic Osteoarthritis in China Caused by Selenium Deficiency.; Authors - Yijie Guo
human	WB	Biol Trace Elem Res; Serious Selenium Deficiency in the Serum of Patients with Kashin-Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes.; Authors - Liyun Wang
rat	IHC	J Trace Elem Med Biol; The effects of long-term low selenium diet on the expression of CHST-3, CHST-12 and UST in knee cartilage of growing rats; Authors - Liyun Wang