CHST8 Polyclonal antibody

• 

  WB analysis of mouse heart using 16426-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16426-1-AP (CHST8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16426-1-AP (CHST8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse skin using 16426-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CHST8 antibody (16426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skin using 16426-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CHST8 antibody (16426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CHST8 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 16426-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

EC:2.8.2.-, GalNAc 4 O sulfotransferase 1, GalNAc 4 ST1, GalNAc-4-O-sulfotransferase 1, GalNAc4ST

---

Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse heart tissue, mouse cerebellum tissue, rat cerebellum tissue
• Positive IF-P detected in: mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16426-1-AP targets CHST8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9669

  Product name: Recombinant human CHST8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 75-424 aa of BC014250

  Sequence: VTRDLSSGAPRGRNLPAPDQPQPPLQRGTRLRLRQRRRRLLIKKMPAAATIPANSSDAPFIRPGPGTLDGRWVSLHRSQQERKRVMQEACAKYRASSSRRAVTPRHVSRIFVEDRHRVLYCEVPKAGCSNWKRVLMVLAGLASSTADIQHNTVHYGSALKRLDTFDRQGILHRLSTYTKMLFVREPFERLVSAFRDKFEHPNSYYHPVFGKAILARYRANASREALRTGSGVRFPEFVQYLLDVHRPVGMDIHWDHVSRLCSPCLIDYDFVGKFESMEDDANFFLSLIRAPRNLTFPRFKDRHSQEARTTARIAHQYFAQLSALQRQRTYDFYYMDYLMFNYSKPFADLY

• Full Name: carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8
• Calculated Molecular Weight: 424 aa, 49 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC014250
• Gene Symbol: CHST8
• Gene ID (NCBI): 64377
• RRID: AB_2229376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2A9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHST8 (Carbohydrate sulfotransferase 8), also named GalNAc-4-O-sulfotransferase 1 (GalNAc4-ST1), is a Golgi-resident type II transmembrane enzyme that transfers sulfate to the C-4 hydroxyl group of terminal β1,4-linked N-acetylgalactosamine (GalNAc) residues present in both N- and O-glycans.