CHSY1 Polyclonal antibody

CHSY1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14420-1-AP

Synonyms

chondroitin sulfate synthase 1, Chondroitin synthase 1, CHSY, ChSy 1, CHSY1, CSS1, KIAA0990



Tested Applications

Positive WB detected inmouse spleen tissue, mouse heart tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14420-1-AP targets CHSY1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHSY1 fusion protein Ag5766
Full Name chondroitin sulfate synthase 1
Calculated molecular weight 92 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC046247
Gene symbol CHSY1
Gene ID (NCBI) 22856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHSY1 antibody 14420-1-APDownload protocol
IHC protocol for CHSY1 antibody 14420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncotarget

Inflammatory microRNA-194 and -515 attenuate the biosynthesis of chondroitin sulfate during human intervertebral disc degeneration.

Authors - Bo Hu
mouseIHC

Front Neurosci

Sleep and Memory Consolidation Dysfunction in Psychiatric Disorders: Evidence for the Involvement of Extracellular Matrix Molecules.

Authors - Barbara Gisabella
humanWB

Am J Hum Genet

Loss of CHSY1, a secreted FRINGE enzyme, causes syndromic brachydactyly in humans via increased NOTCH signaling.

Authors - Tian Jing J