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  • KD/KO Validated

CIB1 Polyclonal antibody

CIB1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11823-1-AP

Synonyms

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28



Tested Applications

Positive WB detected inhuman liver tissue, HepG2 cells, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11823-1-AP targets CIB1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIB1 fusion protein Ag2391
Full Name calcium and integrin binding 1 (calmyrin)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC000846
Gene symbol CIB1
Gene ID (NCBI) 10519
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.

Protocols

Product Specific Protocols
WB protocol for CIB1 antibody 11823-1-APDownload protocol
IHC protocol for CIB1 antibody 11823-1-APDownload protocol
IP protocol for CIB1 antibody 11823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

F1000Res

III. Cellular ultrastructures in situ as key to understanding tumor energy metabolism: biological significance of the Warburg effect.

Authors - Halina Witkiewicz

F1000Res

I. Embryonal vasculature formation recapitulated in transgenic mammary tumor spheroids implanted pseudo-orthotopicly into mouse dorsal skin fold: the organoblasts concept.

Authors - Halina Witkiewicz
mouseWB

Front Mol Neurosci

Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease.

Authors - Magdalena Czeredys
humanWB, IF

J Virol

p130Cas Scaffolds the Signalosome to Direct Adaptor-Effector Crosstalk During Kaposi's Sarcoma-Associated Herpesvirus Trafficking in Human Microvascular Dermal Endothelial Cells.

Authors - Chirosree Bandyopadhyay
  • KD Validated
ratWB

Sci Rep

PDI regulates seizure activity via NMDA receptor redox in rats.

Authors - Ji Yang Kim
mouseWB

FASEB J

Identification of calcium and integrin-binding protein 1 as a novel regulator of production of amyloid β peptide using CRISPR/Cas9-based screening system.

Authors - Yung Wen Chiu
  • KO Validated