CIB1 Recombinant monoclonal antibody

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• CIB1 Recombinant monoclonal antibody (87697-1-RR)

CIB1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87697-1-RR

Clone No.253241A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Calcium- and integrin-binding protein, Calcium and integrin-binding protein 1, Calmyrin, CIB, CIBP

• 

  WB analysis using 87697-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87697-1-RR (CIB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87697-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87697-1-RR against Human CIB1 were performed. The affinity constant is 2.02 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: RT-4 cells, LNCaP cells, K-562 cells, Jurkat cells, HepG2 cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87697-1-RR targets CIB1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5476

  Product name: Recombinant human CIB1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 2-191 aa of NM_006384.3

  Sequence: GGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL

• Full Name: calcium and integrin binding 1 (calmyrin)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_006384.3
• Gene Symbol: CIB1
• Gene ID (NCBI): 10519
• RRID: AB_3745703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99828-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIB1 (Calcium and Integrin-Binding protein 1) is a small, multifunctional calcium-binding protein defined by its four EF-hand domains, which enable its role as a key signaling adaptor within the cell. Originally identified for its ability to bind the cytoplasmic tail of the platelet integrin αIIbβ3, CIB1 has since been shown to interact with a diverse array of partners, including DNA-dependent protein kinase, focal adhesion kinase, and protein kinase D. Through these interactions, CIB1 regulates numerous cellular processes such as cell survival, proliferation, migration, and apoptosis.