CIITA Polyclonal antibody

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  WB analysis of K-562 using 55099-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55099-1-AP (CIITA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CIITA Polyclonal Antibody for WB, ELISA

Cat No. 55099-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C2TA, CIITA, CIITAIV, MHC class II transactivator, MHC2TA, NLRA

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55099-1-AP targets CIITA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: class II, major histocompatibility complex, transactivator
• Calculated Molecular Weight: 124 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_000246
• Gene Symbol: CIITA
• Gene ID (NCBI): 4261
• RRID: AB_2881268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33076
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIITA, also naned as MHC2TA, is a 1130 amino acid protein, which Interacts with ZXDA and ZXDC. MHC class II molecules are heterodimeric cell surface glycoproteins expressed on B cells, macrophages and dendritic cells, which present antigens to CD4+ T cells. CIITA (class II transactivator) acts as a coactivator for MHC class II-specific gene expression and negatively regulates the IL-4 gene promoter during T cell differentiation. IFNg induces CIITA gene expression via JAK1 and Stat1 pathways. T Defects of CIITA and RFX-B have been implicated as causes of Bare Lymphocyte Syndrome (BLS), which is characterized by the absence of MHC class II transcription and severe immunodeficiencies.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Oncol; Identification and validation of m6A-GPI signatures as a novel prognostic model for colorectal cancer; Authors - Bin Ma