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CIN85 Polyclonal antibody

CIN85 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 12132-1-AP


CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1

Tested Applications

Positive WB detected inRaji cells, fetal human brain tissue, Ramos cells, Jurkat cells, THP-1 cells, NIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12132-1-AP targets CIN85 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIN85 fusion protein Ag2777
Full Name SH3-domain kinase binding protein 1
Calculated Molecular Weight 665 aa, 73 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC015806
Gene Symbol SH3KBP1
Gene ID (NCBI) 30011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.


Product Specific Protocols
WB protocol for CIN85 antibody 12132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


humanWB, IF

J Cell Biol

CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.

Authors - Ihor Yakymovych

Biol Cell

ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.

Authors - Yoon Hye-Young HY
  • KD Validated

Arch Gynecol Obstet

Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.

Authors - Ma Yaxi Y