CIP29 Polyclonal antibody

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• CIP29 Polyclonal antibody (15798-1-AP)

CIP29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15798-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Proliferation-associated cytokine-inducible protein CIP29, Nuclear protein Hcc-1, HSPC316, HCC1, Cytokine-induced protein of 29 kDa

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  WB analysis using 15798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of rat pancreas using 15798-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 15798-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 15798-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 15798-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15798-1-AP (CIP29 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 15798-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 15798-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells, rat pancreas tissue, HeLa cells, MCF-7 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15798-1-AP targets CIP29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8507

  Product name: Recombinant human CIP29 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-210 aa of BC007099

  Sequence: MATETVELHKLKLAELKQECLARGLETKGIKQDLIHRLQAYLEEHAEEEANEEDVLGDETEEEETKPIELPVKEEEPPEKTVDVAAEKKVVKITSEIPQTERMQKRAERFNVPVSLESKKAARAARFGISSVPTKGLSSDNKPMVNLDKLKERAQRFGLNVSSISRKSEDDEKLKKRKERFGIVTSSAGTGTTEDTEAKKRKRAERFGIA

• Full Name: cytokine induced protein 29 kDa
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: BC007099
• Gene Symbol: CIP29
• Gene ID (NCBI): 84324
• RRID: AB_1959356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P82979
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CIP29 Polyclonal antibody (Cat# 15798-1-AP) has 3 total citations. These appear in PLoS Pathogens, Frontiers in Cardiovascular Medicine, and Tumour Biology. The associated research fields include influenza A virus polymerase regulation and replication, protein lactylation in ischemic mouse hearts, and epithelial ovarian cancer progression via PDIA3 expression. Applications cited include Western blot and immunofluorescence in human and mouse samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	PLoS Pathog; TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus; Authors - Lingcai Zhao ; KD Validated
mouse	WB	Front Cardiovasc Med; Characterization of protein lactylation in healthy and ischemic mouse hearts.; Authors - Daiqian Wu
human	WB	Tumour Biol; Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.; Authors - Ying Zhu