CIT Polyclonal antibody, PBS Only

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• CIT Polyclonal antibody, PBS Only (20033-1-PBS)

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  WB analysis of A431 using 20033-1-AP (same clone as 20033-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  WB analysis using 20033-1-AP (same clone as 20033-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20033-1-AP (same clone as 20033-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20033-1-AP (same clone as 20033-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using CIT antibody 20033-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

CIT Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20033-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

20033-1-PBS targets CIT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: citron (rho-interacting, serine/threonine kinase 21)
• Calculated Molecular Weight: 231 kDa
• Observed Molecular Weight: 230 kDa, 177 kDa
• GenBank Accession Number: NM_007174
• Gene Symbol: CIT
• Gene ID (NCBI): 11113
• RRID: AB_10665814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14578
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.