Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-3 cells, SGC-7901 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25486-1-AP targets CKAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21901 Product name: Recombinant human CKAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-356 aa of BC136332 Sequence: MSTPAVPQDLQLPPSQRAQSAFKEQRRQKLKEHLLRRKTLFAYKQENEMLSSRDQRVVTSEDQVQEGTKVLKLKTKMADKENMKRPAESKNNTVVGKHCIPLKPSNELTNSTVVIDTHKPKDSNQTPHLLLTEDDPQSQHMTLSQAFHLKNNSKKKQMTTEKQKQDANMPKKPVLGSYRGQIVQSKINSFRKPLQVKDESSAATKKLSATIPKATKPQPVNTSSVTVKSNRSSNMTATTKFVSTTSQNTQLVRPPIRSHHSNTRDTVKQGISRTSANVTIRKGPHEKELLQSKTALSSVKTSSSQGIIRNKTLSRSIASEVVARPASLSNDKLMEKSEPVDQRRHTAGKAIVDSRS Predict reactive species |
| Full Name | cytoskeleton associated protein 2 |
| Calculated Molecular Weight | 683 aa, 77 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | BC136332 |
| Gene Symbol | CKAP2 |
| Gene ID (NCBI) | 26586 |
| RRID | AB_2880100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWK9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated protein (TMAP), is implicated in the regulation of cell proliferation (PMID: 30119212). CKAP2 is a MAP expressed during mitosis that localizes to centrosomes and spindles before being targeted for degradation by the anaphase-promoting complex/cyclosome at mitotic exit (PMID: 38381793, 17339342).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CKAP2 antibody 25486-1-AP | Download protocol |
| IHC protocol for CKAP2 antibody 25486-1-AP | Download protocol |
| WB protocol for CKAP2 antibody 25486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CKAP2 Polyclonal antibody (Cat# 25486-1-AP) has 11 citations. These appear in journals including Nature Cell Biology, Molecular Cell, Advanced Science, PNAS, Frontiers in Immunology, Molecules, Cancers, Oncology Reports, bioRxiv, Translational Oncology, and World Journal of Surgical Oncology. Research fields span cell biology and spindle architecture, RNA m6A translation, kinase reprogramming, microtubule dynamics, and cancer—covering rheumatoid arthritis, gastric, breast, glioma, ovarian, and bladder cancers. Applications are primarily Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Cell state-specific cytoplasmic density controls spindle architecture and scaling | ||
Mol Cell The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance
| ||
Adv Sci (Weinh) Covalent Reprogramming of Kinase Binders to Modulate Protein Abundance.
| ||
Proc Natl Acad Sci U S A The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregation | ||
Front Immunol Identification and validation of CKAP2 as a novel biomarker in the development and progression of rheumatoid arthritis | ||
Molecules Integrated Single-Cell Virtual Knockout and Machine Learning Analyses Reveal a Protective Role of CKAP2 in Gastric Cancer. |
Reviews
What customers say
The single review reports a 5-star experience using this antibody for immunofluorescence on HT-1080 human fibrosarcoma cells. The user applied a 1/400 dilution with an overnight incubation and found the results excellent, noting it "worked great" for the application. Overall, the feedback is positive, highlighting reliable performance in IF with straightforward protocol conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Azriel (Verified Customer) (12-02-2019) | Worked great for IF of HT-1080 cells at a 1/400 dilution incubation overnight
|
