CKAP2 Polyclonal antibody

CKAP2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 25486-1-AP

Synonyms

CKAP2, CTCL tumor antigen se20 10, LB1, se20 10, TMAP



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, PC-3 cells, SGC-7901 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25486-1-AP is CKAP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKAP2 fusion protein Ag21901
Full Name cytoskeleton associated protein 2
Calculated molecular weight 683 aa, 77 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC136332
Gene symbol CKAP2
Gene ID (NCBI) 26586
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CKAP2 antibody 25486-1-APDownload protocol
IF protocol for CKAP2 antibody 25486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high‑grade glioma.

Authors - Kuanyu Wang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azriel (Verified Customer) (12-02-2019)

Worked great for IF of HT-1080 cells at a 1/400 dilution incubation overnight

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/400
  • Cell Tissue Type: HT-1080 human fibrosarcoma cell line