CKAP2 Polyclonal antibody

• 

  WB analysis of HeLa using 25486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 25486-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25486-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25486-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 25486-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25486-1-AP (CKAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CKAP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25486-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Cytoskeleton-associated protein 2, LB1, se20-10, TMAP, Tumor- and microtubule-associated protein

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, PC-3 cells, SGC-7901 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Product Information

25486-1-AP targets CKAP2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21901

  Product name: Recombinant human CKAP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-356 aa of BC136332

  Sequence: MSTPAVPQDLQLPPSQRAQSAFKEQRRQKLKEHLLRRKTLFAYKQENEMLSSRDQRVVTSEDQVQEGTKVLKLKTKMADKENMKRPAESKNNTVVGKHCIPLKPSNELTNSTVVIDTHKPKDSNQTPHLLLTEDDPQSQHMTLSQAFHLKNNSKKKQMTTEKQKQDANMPKKPVLGSYRGQIVQSKINSFRKPLQVKDESSAATKKLSATIPKATKPQPVNTSSVTVKSNRSSNMTATTKFVSTTSQNTQLVRPPIRSHHSNTRDTVKQGISRTSANVTIRKGPHEKELLQSKTALSSVKTSSSQGIIRNKTLSRSIASEVVARPASLSNDKLMEKSEPVDQRRHTAGKAIVDSRS

• Full Name: cytoskeleton associated protein 2
• Calculated Molecular Weight: 683 aa, 77 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC136332
• Gene Symbol: CKAP2
• Gene ID (NCBI): 26586
• RRID: AB_2880100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WWK9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated protein (TMAP), is implicated in the regulation of cell proliferation (PMID: 30119212). CKAP2 is a MAP expressed during mitosis that localizes to centrosomes and spindles before being targeted for degradation by the anaphase-promoting complex/cyclosome at mitotic exit (PMID: 38381793, 17339342).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregation; Authors - Lia Mara Gomes Paim
human	WB	Oncol Rep; CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high‑grade glioma.; Authors - Kuanyu Wang ; KD Validated
human	WB,IF	Cancers (Basel); Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer; Authors - Alexsandro dos Santos
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu ; KD Validated
mouse	WB	Nat Cell Biol; Cell state-specific cytoplasmic density controls spindle architecture and scaling; Authors - Tobias Kletter

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Azriel (Verified Customer) (12-02-2019)

Worked great for IF of HT-1080 cells at a 1/400 dilution incubation overnight

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: HT-1080 human fibrosarcoma cell line