CKM/CKB Polyclonal antibody

CKM/CKB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15891-1-AP

Synonyms

CKM, CKM/CKB, CKMM, Creatine Kinase, Creatine Kinase MM, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK



Tested Applications

Positive WB detected inmouse heart tissue, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15891-1-AP targets CKM/CKB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKM/CKB fusion protein Ag8678
Full Name creatine kinase, muscle
Calculated Molecular Weight 381 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC007462
Gene Symbol CKM
Gene ID (NCBI) 1158
RRIDAB_2260647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody can recognize both CKB and CKM due to the high homology.

Protocols

Product Specific Protocols
WB protocol for CKM/CKB antibody 15891-1-APDownload protocol
IHC protocol for CKM/CKB antibody 15891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Lipids Health Dis

Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.

Authors - David W Russ
humanWB

Spinal Cord

The relationship between skin ultrasound images and muscle damage using skin blotting in wheelchair basketball athletes.

Authors - Nao Tamai
humanWB

Nanomedicine

Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer.

Authors - Adel Al Fatease