Tested Applications
| Positive WB detected in | human brain tissue, HEK-293 cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12942-1-AP targets CLASP2 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4012 Product name: Recombinant human CLASP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-308 aa of BC029035 Sequence: MRRLICKRICDYKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSARKPGSAGGPKVGGASKEGGAGAVDEDDFIKAFTDVPSIQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQK Predict reactive species |
| Full Name | cytoplasmic linker associated protein 2 |
| Calculated Molecular Weight | 1294 aa, 141 kDa |
| Observed Molecular Weight | 140-160 kDa |
| GenBank Accession Number | BC029035 |
| Gene Symbol | CLASP2 |
| Gene ID (NCBI) | 23122 |
| RRID | AB_2260448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75122 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLASP2 (CLIP-associating protein 2) is a brain specific protein belongs to CLASP family. CLASP1 and CLASP2 regulate microtubule plus-end dynamics in brine cortex. CLASP2 is required for the orientation of stabilized microtubules toward the leading edge, It may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms. Phosphorylation of CLASP2 by GSK3B may negatively regulate binding to microtubule lattices in lamella.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CLASP2 antibody 12942-1-AP | Download protocol |
| WB protocol for CLASP2 antibody 12942-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CLASP2 Polyclonal antibody (Cat# 12942-1-AP) has 1 citation. It appears in the Journal of Bone and Mineral Research (2013), in a study on protein kinase B-mediated microtubule stabilization regulating bone resorption and sealing zone formation in osteoclasts. The research field encompasses bone biology, osteoclast function, and microtubule dynamics. The citation used the antibody for Western blot in mouse samples.
| Species | Application | Title |
|---|---|---|
J Bone Miner Res Regulation of bone resorption and sealing zone formation in osteoclasts occurs through protein kinase B-mediated microtubule stabilization. |
Reviews
What customers say
The single review reports successful use of this antibody for Western Blot at a 1:500 dilution on HEK 293T cells. The reviewer loaded 30 µg of HEK protein and detected CLASP2 levels in both control cells and cells transfected with CLASP2 siRNA, confirming the antibody's ability to recognize the target and detect knockdown. The product received a 4-star rating. Overall, the feedback indicates reliable performance for CLASP2 detection in Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (07-06-2020) | 30ug HEK protein loaded, and CLASP2 levels detected for control cells or cells transfected with CLASP2 siRNA
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