CLCA2 Polyclonal antibody

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• CLCA2 Polyclonal antibody (19273-1-AP)

CLCA2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19273-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

CACC, CaCC 3, CACC3, CaCC-3, Calcium-activated chloride channel family member 2

• 

  WB analysis of mouse lung using 19273-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19273-1-AP (CLCA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 19273-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19273-1-AP (CLCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human oesophagus using 19273-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 19273-1-AP (CLCA2 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human oesophagus using 19273-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 19273-1-AP (CLCA2 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse lung tissue, COLO 320 cells
• Positive IHC detected in: human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19273-1-AP targets CLCA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5914

  Product name: Recombinant human CLCA2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 595-901 aa of BC041096

  Sequence: SRASNSAVPPATVEAFVERDSLHFPHPVMIYANVKQGFYPILNATVTATVEPETGDPVTLRLLDDGAGADVIKNDGIYSRYFFSFAANGRYSLKVHVNHSPSISTPAHSIPGSHAMYVPGYTANGNIQMNAPRKSVGRNEEERKWGFSRVSSGGSFSVLGVPAGPHPDVFPPCKIIDLEAVKVEEELTLSWTAPGEDFDQGQATSYEIRMSKSLQNIQDDFNNAILVNTSKRNPQQAGIREIFTFSPQISTNGPEHQPNGETHESHRIYVAIRAMDRNSLQSAVSNIAQAPLFIPPNSDPVPARDYL

• Full Name: chloride channel accessory 2
• Calculated Molecular Weight: 104 kDa
• Observed Molecular Weight: 141 kDa, 35-40 kDa
• GenBank Accession Number: BC041096
• Gene Symbol: CLCA2
• Gene ID (NCBI): 9635
• RRID: AB_10642000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UQC9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-CLCA2 (Chloride Channel Accessory 2) antibody, catalog number 19273-1-AP, has 4 total citations. These publications appear in Frontiers in Cell and Developmental Biology, Journal of Ovarian Research, Biomedicines, and Frontiers in Cardiovascular Medicine. The associated research fields include cardiac fibrosis, premature ovarian insufficiency and apoptosis, chloride channel self-cleavage and store-operated calcium entry regulation, and post-ischemia heart regeneration.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IP	Front Cell Dev Biol; Epigenetic Repression of Chloride Channel Accessory 2 Transcription in Cardiac Fibroblast: Implication in Cardiac Fibrosis.; Authors - Tinghui Shao
human	WB	J Ovarian Res; TP63 truncating mutation causes increased cell apoptosis and premature ovarian insufficiency by enhanced transcriptional activation of CLCA2; Authors - Yali Fan ; KD Validated
human	IP	Biomedicines; Self-Cleavage of Human Chloride Channel Accessory 2 Causes a Conformational Shift That Depends on Membrane Anchorage and Is Required for Its Regulation of Store-Operated Calcium Entry; Authors - Grace T Ramena
mouse	WB	Front Cardiovasc Med; Microenvironment Stiffness Amplifies Post-ischemia Heart Regeneration in Response to Exogenous Extracellular Matrix Proteins in Neonatal Mice.; Authors - Xinming Wang