CLCA2 Polyclonal antibody

CLCA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19273-1-AP

Synonyms

CACC, CaCC 3, CACC3, chloride channel accessory 2, CLCA2, CLCRG2, hCaCC 3, hCLCA2



Tested Applications

Positive WB detected inmouse lung tissue, COLO 320 cells
Positive IHC detected inhuman oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19273-1-AP is CLCA2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCA2 fusion protein Ag5914
Full Name chloride channel accessory 2
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 141 kDa, 35-40 kDa
GenBank Accession NumberBC041096
Gene Symbol CLCA2
Gene ID (NCBI) 9635
RRIDAB_10642000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLCA2 antibody 19273-1-APDownload protocol
IHC protocol for CLCA2 antibody 19273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Front Cell Dev Biol

Epigenetic Repression of Chloride Channel Accessory 2 Transcription in Cardiac Fibroblast: Implication in Cardiac Fibrosis.

Authors - Tinghui Shao
mouseWB

Front Cardiovasc Med

Microenvironment Stiffness Amplifies Post-ischemia Heart Regeneration in Response to Exogenous Extracellular Matrix Proteins in Neonatal Mice.

Authors - Xinming Wang
humanIP

Biomedicines

Self-Cleavage of Human Chloride Channel Accessory 2 Causes a Conformational Shift That Depends on Membrane Anchorage and Is Required for Its Regulation of Store-Operated Calcium Entry

Authors - Grace T Ramena
humanWB

J Ovarian Res

TP63 truncating mutation causes increased cell apoptosis and premature ovarian insufficiency by enhanced transcriptional activation of CLCA2

Authors - Yali Fan
  • KD Validated