CLCN5 Polyclonal antibody

CLCN5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26812-1-AP

Synonyms

chloride channel 5, Chloride channel protein 5, Chloride transporter ClC 5, ClC 5, CLC5, CLCK2, CLCN5, DENTS, hCIC K2, hClC K2, XLRH, XRN

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Validation Data Gallery

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  WB analysis of MDCK using 26812-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 26812-1-AP (CLCN5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26812-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26812-1-AP (CLCN5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 26812-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26812-1-AP (CLCN5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26812-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26812-1-AP (CLCN5 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: MDCK cells, mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26812-1-AP is CLCN5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, Canine, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLCN5 fusion protein Ag25182
• Full Name: chloride channel 5
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC130429
• Gene Symbol: CLCN5
• Gene ID (NCBI): 1184
• RRID: AB_2880643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Gene Ther; Gene therapy of Dent disease type 1 in newborn ClC-5 null mice for sustained transgene expression and gene therapy effects; Authors - Pin Lyu