Claudin 3 Polyclonal antibody
Claudin 3 Polyclonal Antibody for IHC, IP,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16456-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IP detected in | mouse large intestine tissue |
| Positive IHC detected in | human breast cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IHC | See 8 publications below |
| IF | See 9 publications below |
Product Information
16456-1-AP targets Claudin 3 in IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, sheep, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Claudin 3 fusion protein Ag9411 |
| Full Name | claudin 3 |
| Calculated molecular weight | 220 aa, 23 kDa |
| Observed molecular weight | 20-24 kDa |
| GenBank accession number | BC016056 |
| Gene symbol | CLDN3 |
| Gene ID (NCBI) | 1365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Claudin 3 antibody 16456-1-AP | Download protocol |
| IP protocol for Claudin 3 antibody 16456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging Cell Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts. | ||
EMBO Mol Med Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation. | ||
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
Environ Int Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments. | ||
NanoImpact Altered protein S-glutathionylation depicts redox imbalance triggered by transition metal oxide nanoparticles in a breastfeeding system. | ||
Mol Nutr Food Res Curcumin Alleviates Dextran Sulfate Sodium-induced Colitis in Mice Through Regulating Gut Microbiota. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Priya (Verified Customer) (07-31-2023) | Used for Caco2 cells and mice tissue
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