Claudin 3 Polyclonal antibody
Claudin 3 Polyclonal Antibody for IHC, IP,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
IP, IHC, IF, ELISA
Cat no : 16456-1-AP
|Positive IP detected in||mouse large intestine tissue|
|Positive IHC detected in||human breast cancer tissue, mouse colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 17 publications below|
|IHC||See 6 publications below|
|IF||See 6 publications below|
16456-1-AP targets Claudin 3 in IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Claudin 3 fusion protein Ag9411|
|Full Name||claudin 3|
|Calculated molecular weight||220 aa, 23 kDa|
|Observed molecular weight||20-24 kDa|
|GenBank accession number||BC016056|
|Gene ID (NCBI)||1365|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
|Product Specific Protocols|
|IHC protocol for Claudin 3 antibody 16456-1-AP||Download protocol|
|IP protocol for Claudin 3 antibody 16456-1-AP||Download protocol|
|Click here to view our Standard Protocols|
EMBO Mol Med
Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.
J Hazard Mater
Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.
Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments.
Altered protein S-glutathionylation depicts redox imbalance triggered by transition metal oxide nanoparticles in a breastfeeding system.
Mol Nutr Food Res
Curcumin Alleviates Dextran Sulfate Sodium-induced Colitis in Mice Through Regulating Gut Microbiota.
JAC4 Protects from X-ray Radiation-Induced Intestinal Injury by JWA-Mediated Anti-Oxidation/Inflammation Signaling.