Tested Applications
| Positive IP detected in | mouse large intestine tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16456-1-AP targets Claudin 3 in IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, bovine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9411 Product name: Recombinant human Claudin 3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-220 aa of BC016056 Sequence: MSMGLEITGTALAVLGWLGTIVCCALPMWRVSAFIGSNIITSQNIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALIVVAILLAAFGLLVALVGAQCTNCVQDDTAKAKITIVAGVLFLLAALLTLVPVSWSANTIIRDFYNPVVPEAQKREMGAGLYVGWAAAALQLLGGALLCCSCPPREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV Predict reactive species |
| Full Name | claudin 3 |
| Calculated Molecular Weight | 220 aa, 23 kDa |
| Observed Molecular Weight | 20-24 kDa |
| GenBank Accession Number | BC016056 |
| Gene Symbol | Claudin 3 |
| Gene ID (NCBI) | 1365 |
| RRID | AB_10733226 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15551 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Claudin 3 antibody 16456-1-AP | Download protocol |
| IP protocol for Claudin 3 antibody 16456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Claudin 3 polyclonal antibody (Cat# 16456-1-AP) has 58 citations spanning journals such as Nature Microbiology, Cell Reports, EMBO Molecular Medicine, Oncogene, Environmental International, Journal of Hazardous Materials, and Food & Function. Research fields include intestinal barrier integrity and inflammation, gut microbiota modulation, blood-brain barrier repair, mammary gland biology, environmental toxicology (nanoparticles, mycotoxins), cancer progression, neuroprotection, and infectious disease.
| Species | Application | Title |
|---|---|---|
J Adv Res Live and pasteurized Akkermansia muciniphila decrease susceptibility to Salmonella Typhimurium infection in mice | ||
J Nanobiotechnology Platelet-rich plasma-derived exosomes promote blood-spinal cord barrier repair and attenuate neuroinflammation after spinal cord injury | ||
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
J Hazard Mater Insight into perfluorooctanoic acid-induced impairment of mouse embryo implantation via single-cell RNA-seq | ||
Environ Int Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments. |
Reviews
What customers say
Both reviewers (the same user) gave 5-star ratings, using the antibody at a 1:1000 dilution for Western Blot on Caco2 cells and mouse colon tissue. The results were satisfactory across two different lots (00013446 and 00007923), indicating consistent performance between batches. Overall, users report reliable performance for WB applications in both cell lines and tissue samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Priya (Verified Customer) (07-31-2023) | Used for Caco2 cells and mice tissue
|
Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells andmice tissue
|
