Claudin 5 Polyclonal antibody

Claudin 5 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13805-1-AP

Synonyms

AWAL, BEC1, claudin 5, CLDN5, CPETRL1, TMDVCF, TMVCF

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Validation Data Gallery

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 3 publications below

Product Information

13805-1-AP targets Claudin 5 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 5 fusion protein Ag4799
• Full Name: claudin 5
• Calculated Molecular Weight: 218 aa, 23 kDa
• GenBank Accession Number: BC032363
• Gene Symbol: CLDN5
• Gene ID (NCBI): 7122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publications

Species	Application	Title
rat	IF	Sci Rep; A dynamic in vivo-like organotypic blood-brain barrier model to probe metastatic brain tumors.; Authors - Hui Xu
mouse	WB,IF	Mol Neurobiol; bFGF Protects Against Blood-Brain Barrier Damage Through Junction Protein Regulation via PI3K-Akt-Rac1 Pathway Following Traumatic Brain Injury.; Authors - Zhou-Guang Wang
human,mouse	WB	Front Pharmacol; ALG-bFGF Hydrogel Inhibiting Autophagy Contributes to Protection of Blood-Spinal Cord Barrier Integrity via PI3K/Akt/FOXO1/KLF4 Pathway After SCI.; Authors - Renkan Zhang
rat	WB	Molecules; Neuroprotective Effects of Rhynchophylline Against Ischemic Brain Injury via Regulation of the Akt/mTOR and TLRs Signaling Pathways.; Authors - Houcai Huang
rat	WB	Biochem Biophys Res Commun; Activation of G protein-coupled estrogen receptor 1 (GPER-1) ameliorates blood-brain barrier permeability after global cerebral ischemia in ovariectomized rats.; Authors - Dan Lu
human	WB	Mol Ther Nucleic Acids; Role of linc00174/miR-138-5p (miR-150-5p)/FOSL2 Feedback Loop on Regulating the Blood-Tumor Barrier Permeability.; Authors - Jizhe Guo