CLEC10A/CD301 Polyclonal antibody

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  WB analysis of BxPC-3 using 13590-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13590-1-AP (CLEC10A/CD301 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

CLEC10A/CD301 Polyclonal Antibody for WB, ELISA

Cat No. 13590-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CLEC10A, CD301, C-type lectin superfamily member 14, C-type lectin domain family 10 member A, CLECSF14

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Tested Applications

• Positive WB detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13590-1-AP targets CLEC10A/CD301 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLEC10A/CD301 fusion protein Ag4490
• Full Name: C-type lectin domain family 10, member A
• Calculated Molecular Weight: 316 aa, 35 kDa
• Observed Molecular Weight: 32 kDa, 28 kDa
• GenBank Accession Number: BC039011
• Gene Symbol: CLEC10A
• Gene ID (NCBI): 10462
• RRID: AB_2083131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IUN9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).