CLEC12A Recombinant monoclonal antibody

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  WB analysis of U-937 using 83680-5-RR

  U-937 cells were subjected to SDS PAGE followed by western blot with 83680-5-RR (CLEC12A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83680-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83680-5-RR against Human CLEC12A were performed. The affinity constant is below 1 pM.

CLEC12A Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83680-5-RR

Clone No.240791C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CLL-1, CLL1, CLL 1, CD371, C type lectin like molecule 1

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Tested Applications

• Positive WB detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83680-5-RR targets CLEC12A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: C-type lectin domain family 12, member A
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 55-75 kDa
• GenBank Accession Number: BC126291
• Gene Symbol: CLEC12A
• Gene ID (NCBI): 160364
• RRID: AB_3671282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5QGZ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).