CLEC18A Polyclonal antibody

CLEC18A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21013-1-AP

Synonyms

MRLP2, MRCL1, MRCL, Mannose receptor-like protein 2, C-type lectin domain family 18 member A

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Validation Data Gallery

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  WB analysis of HeLa using 21013-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21013-1-AP (CLEC18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21013-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21013-1-AP (CLEC18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21013-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21013-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 21013-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 21013-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21013-1-AP targets CLEC18A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLEC18A fusion protein Ag15237
• Full Name: C-type lectin domain family 18, member A
• Calculated Molecular Weight: 446 aa, 50 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC141808
• Gene Symbol: CLEC18A
• Gene ID (NCBI): 348174
• RRID: AB_10732842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC18A, or C-type lectin member 18A, is a protein that plays a significant role in the human immune system, particularly in the context of viral infections. CLEC18A is widely expressed, with the highest levels found in myeloid cells and the liver. It is a co-receptor for TLR3 (Toll-like receptor 3) and is involved in enhancing the host's immune response to viral infections, such as H5N1 influenza A virus (IAV). CLEC18A has been implicated in the development of mixed cryoglobulinemia (MC), an extrahepatic manifestation associated with hepatitis C virus (HCV). This antibody can recognize CLEC18A/B/C.