Tested Applications
| Positive WB detected in | human plasma tissue |
| Positive IP detected in | human plasma tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20311-1-AP targets Tetranectin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14147 Product name: Recombinant human CLEC3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 78-202 aa of BC011024 Sequence: HMKCFLAFTQTKTFHEASEDCISRGGTLSTPQTGSENDALYEYLRQSVGNEAEIWLGLNDMAAEGTWVDMTGARIAYKNWETEITAQPDGGKTENCAVLSGAANGKWFDKRCRDQLPYICQFGIV Predict reactive species |
| Full Name | C-type lectin domain family 3, member B |
| Calculated Molecular Weight | 202 aa, 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC011024 |
| Gene Symbol | CLEC3B |
| Gene ID (NCBI) | 7123 |
| RRID | AB_2878670 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05452 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C-Type Lectin Domain Family 3 Member B (CLEC3B) is a transmembrane Ca2+-binding protein, locating in cell plasma, extracellular matrix and exosomes. Downregulation of CLEC3B has been reported in various diseases. It was demonstrated in CLEC3B-deficient mice that the absence of CLEC3B impeded the mineralization process in osteogenesis. (PMID: 31477130)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Tetranectin antibody 20311-1-AP | Download protocol |
| IP protocol for Tetranectin antibody 20311-1-AP | Download protocol |
| WB protocol for Tetranectin antibody 20311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CLEC3B Polyclonal Antibody (Cat# 20311-1-AP) has 3 citations published in Materials Today Bio, Journal of Translational Medicine, and European Journal of Medical Research. The research fields span non-small cell lung cancer immunotherapy, tumor microenvironment remodeling by cancer-associated fibroblasts in lung adenocarcinoma, and triple-negative breast cancer chemoresistance via ferroptosis induction. Applications cited include IHC, IP, and WB in both human and mouse samples.
| Species | Application | Title |
|---|---|---|
Mater Today Bio Integrating gene demethylation and immune modulation: PD-1 nanovesicles as a dual-action therapy for NSCLC | ||
J Transl Med Decoding the tumor microenvironment remodeling orchestrated by CLEC3B+ inflammatory cancer-associated fibroblasts in lung adenocarcinoma immunotherapy: elucidation from pan-cancer spatially single-cell transcriptomics landscape. | ||
Eur J Med Res C-type lectin domain family 3 member B (CLEC3B) inhibits triple-negative breast cancer chemoresistance via inducing ferroptosis.
|
