Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55451-1-AP targets CLEC9A in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | C-type lectin domain family 9, member A |
| Calculated Molecular Weight | 27 kDa |
| GenBank Accession Number | NM_207345 |
| Gene Symbol | CLEC9A |
| Gene ID (NCBI) | 283420 |
| RRID | AB_2881338 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXN8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLEC9A (C-type lectin domain family 9 member A), also known as DNGR1, CD370, is a type II membrane protein that contains C-type lectin domain. The C-type lectin domain family members share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. Mouse Clec9A is primarily restricted to dendritic cells (DCs) and selectively expressed in mouse CD8+ conventional DCs (cDCs) and plasmacytoid DCs (pDCs), whereas human CLEC9A is also expressed on human DC subtypes. CLEC9A functions as an activating receptor that recruits Syk kinase and can induce the production of proinflammatory cytokines. (PMID: 25553393; PMID: 15476922; PMID: 18669894; PMID: 18408006)
Publications
What published studies show
The CLEC9A Polyclonal antibody (Cat# 55451-1-AP) has 1 total citation. It was cited in Nature Communications (IF 18.1) in a 2026 study on ultra-precision deconvolution of spatial transcriptomics, which decodes immune heterogeneity and fate-defining programs in tissues. The research field encompasses spatial transcriptomics, immunology, and tissue biology. The antibody was applied in IHC for human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Ultra-precision deconvolution of spatial transcriptomics decodes immune heterogeneity and fate-defining programs in tissues. |
Reviews
What customers say
The product has one user review rated 4 out of 5 stars. The reviewer used the antibody for immunohistochemistry on human tonsil FFPE tissue with citrate buffer-based heat-induced epitope retrieval at a 1:200 dilution, reporting satisfactory staining results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Santhosh (Verified Customer) (04-15-2019) | Human Tonsil FFPE tissue. HIER with citrate buffer.
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