CLPP Monoclonal antibody

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  WB analysis of human heart using 66271-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66271-1-Ig (CLPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 66271-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66271-1-Ig (CLPP Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66271-1-Ig (CLPP Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

CLPP Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66271-1-Ig

Clone No.2E1D9

Publications(4)

Host / Isotype

Mouse / IgG3

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

2E1D9, ATP-dependent Clp protease proteolytic subunit, mitochondrial, EC:3.4.21.92, Endopeptidase Clp

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Tested Applications

• Positive WB detected in: human heart tissue, pig heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

66271-1-Ig targets CLPP in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: CLPP fusion protein Ag8420
• Full Name: ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 26 kDa, 30 kDa
• GenBank Accession Number: BC002956
• Gene Symbol: CLPP
• Gene ID (NCBI): 8192
• RRID: AB_2881656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16740
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Publications

Species	Application	Title
human	WB	Nature; Enhancing mitochondrial proteostasis reduces amyloid-β proteotoxicity.; Authors - Vincenzo Sorrentino
human	IF	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
mouse	WB	PeerJ; Mitochondrial unfolded protein response gene CLPP changes mitochondrial dynamics and affects mitochondrial function.; Authors - GuiJun Wu ; KD Validated
human	WB	NeuroImmune Pharm Ther; Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages; Authors - Santosh Kumar