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CLPP Antibody 1 Publications

Mouse Monoclonal| Catalog number: 66271-1-Ig |CloneNo.: 2E1D9

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

CLPP Antibody WB human heart tissue 66271-1-Ig

human heart tissue were subjected to SDS PAGE followed by western blot with 66271-1-Ig( CLPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CLPP Antibody IHC human heart tissue 66271-1-Ig

Immunohistochemistry of paraffin-embedded human heart tissue slide using 66271-1-Ig( CLPP Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, pig
Positive WB detected in:
human heart tissue, pig heart tissue
Positive IHC detected in:
human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:400

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG3
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CLPP fusion protein Ag8420
Full name:
ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
Calculated molecular weight:
30 kDa
Observed molecular weight:
30 kDa
GenBank accession number:
BC002956
Gene ID (NCBI):
8192
Gene symbol
CLPP
Synonyms
CLPP, Endopeptidase Clp

Background

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.


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