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CLPP Monoclonal antibody

CLPP Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, pig and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2E1D9

Cat no : 66271-1-Ig

Synonyms

CLPP, Endopeptidase Clp



Tested Applications

Positive WB detected inhuman heart tissue, pig heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

66271-1-Ig targets CLPP in WB, IHC,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen CLPP fusion protein Ag8420
Full Name ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC002956
Gene symbol CLPP
Gene ID (NCBI) 8192
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Protocols

Product Specific Protocols
WB protocol for CLPP antibody 66271-1-IgDownload protocol
IHC protocol for CLPP antibody 66271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Enhancing mitochondrial proteostasis reduces amyloid-β proteotoxicity.

Authors - Vincenzo Sorrentino
mouseWB

PeerJ

Mitochondrial unfolded protein response gene CLPP changes mitochondrial dynamics and affects mitochondrial function.

Authors - GuiJun Wu
  • KD Validated