|Positive WB detected in||human heart tissue, pig heart tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
66271-1-Ig targets CLPP in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG3|
|Immunogen||CLPP fusion protein Ag8420|
|Full Name||ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)|
|Calculated molecular weight||30 kDa|
|Observed molecular weight||26 kDa, 30 kDa|
|GenBank accession number||BC002956|
|Gene ID (NCBI)||8192|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.
Enhancing mitochondrial proteostasis reduces amyloid-β proteotoxicity.
Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.
Mitochondrial unfolded protein response gene CLPP changes mitochondrial dynamics and affects mitochondrial function.