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CLPS Recombinant monoclonal antibody, PBS Only (Detector) (84224-2-PBS)

Filter: AllWBIHCIF-PCytometric bead arrayAffinity

WB analysis of rat pancreas using 84224-2-RR (same clone as 84224-2-PBS)
rat pancreas tissue were subjected to SDS PAGE followed by western blot with 84224-2-RR (CLPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.
IF Staining of mouse pancreas using 84224-2-RR (same clone as 84224-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CLPS antibody (84224-2-RR, Clone: 241565H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CLPS antibody (84224-2-RR, Clone: 241565H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84224-2-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP01160-1
Cytometric bead array standard curve of MP01160-1, CLPS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84224-1-PBS. Detection antibody: 84224-2-PBS. Standard: Ag2321. Range: 0.781-100 ng/mL.
Affinity and Kinetic Characterization of 84224-2-RR
Biolayer interferometry (BLl) kinetic assays of 84224-2-RR against Human CLPS were performed. The affinity constant is below 1 pM.

CLPS Recombinant monoclonal antibody, PBS Only (Detector)

Name: CLPS Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 84224-2-PBS

CLPS Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 84224-2-PBS

Clone No.241565H3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

Colipase, 241565H3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

84224-2-PBS targets CLPS as part of a matched antibody pair:

MP01160-1: 84224-1-PBS capture and 84224-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2321 Product name: Recombinant human CLPS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-112 aa of BC017897 Sequence: MEKILILLLVALSVAYAAPGPRGIIINLENGELCMNSAQCKSNCCQHSSALGLARCTSMASENSECSVKTLYGIYYKCPCERGLTCEGDKTIVGSITNTNFGICHDAGRSKQ Predict reactive species
Full Name	colipase, pancreatic
Calculated Molecular Weight	12 kDa
Observed Molecular Weight	12 kDa
GenBank Accession Number	BC017897
Gene Symbol	CLPS
Gene ID (NCBI)	1208
RRID	AB_3671777
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P04118
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.