CLTCL1 Polyclonal antibody

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  IP experiment of HeLa using 22283-1-AP

  IP result of anti-CLTCL1 (IP:22283-1-AP, 4ug; Detection:22283-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human testis using 22283-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22283-1-AP (CLTCL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22283-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22283-1-AP (CLTCL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 22283-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22283-1-AP (CLTCL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 22283-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22283-1-AP (CLTCL1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 22283-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLTCL1 antibody (22283-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594-conjugated p115, USO1 antibody (CL594-13509, red).

• Featured Product
• KD/KO Validated

CLTCL1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Cat No. 22283-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CLTD, CLTCL, CLH-22, CLH22, Clathrin heavy chain on chromosome 22

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Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

22283-1-AP targets CLTCL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: clathrin, heavy chain-like 1
• Calculated Molecular Weight: 187 kDa
• Observed Molecular Weight: 180-210 kDa
• GenBank Accession Number: NM_007098
• Gene Symbol: CLTCL1
• Gene ID (NCBI): 8218
• RRID: AB_11183764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53675
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLTCL1, also called CLH22, CLTCL, and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross-reaction to CLTC.

Publications

Species	Application	Title
human	WB,IF	EMBO J; CHC22 clathrin recruitment to the early secretory pathway requires two-site interaction with SNX5 and p115; Authors - Joshua Greig
human	IF,IP	J Cell Biol; CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis.; Authors - Stéphane M Camus ; KD Validated
		Elife; Genetic diversity of CHC22 clathrin impacts its function in glucose metabolism.; Authors - Matteo Fumagalli
human	WB	Proteome Sci; LC-MS-based quantitation of proteomic changes induced by Norcantharidin in MTB-Treated macrophages; Authors - Yi-Lin Wu