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  • KD/KO Validated

CLTCL1 Polyclonal antibody

CLTCL1 Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 22283-1-AP


CHC22, Clathrin heavy chain 2, clathrin, heavy chain like 1, CLH 22, CLH22, CLTCL, CLTCL1, CLTD

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22283-1-AP targets CLTCL1 in IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name clathrin, heavy chain-like 1
Calculated Molecular Weight 187 kDa
Observed Molecular Weight 180-210 kDa
GenBank Accession NumberNM_007098
Gene Symbol CLTCL1
Gene ID (NCBI) 8218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC.


Product Specific Protocols
IHC protocol for CLTCL1 antibody 22283-1-APDownload protocol
IF protocol for CLTCL1 antibody 22283-1-APDownload protocol
IP protocol for CLTCL1 antibody 22283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Cell Biol

CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis.

Authors - Stéphane M Camus
  • KD Validated


Genetic diversity of CHC22 clathrin impacts its function in glucose metabolism.

Authors - Matteo Fumagalli