CLUAP1 Polyclonal antibody

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  WB analysis of mouse testis using 17470-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17470-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 17470-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17470-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17470-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17470-1-AP

  IP result of anti-CLUAP1 (IP:17470-1-AP, 4ug; Detection:17470-1-AP 1:600) with HepG2 cells lysate 2400ug.

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  IF Staining of MDCK using 17470-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CLUAP1 antibody (17470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

• Featured Product
• KD/KO Validated

CLUAP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 17470-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Qilin, KIAA0643, Clusterin-associated protein 1, clusterin associated protein 1

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Tested Applications

• Positive WB detected in: mouse testis tissue, COLO 320 cells, HepG2 cells, Y79 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

17470-1-AP targets CLUAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11419

  Product name: Recombinant human CLUAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-413 aa of BC017070

  Sequence: MSFRDLRNFTEMMRALGYPRHISMENFRTPNFGLVSEVLLWLVKRYEPQTDIPPDVDTEQDRVFFIKAIAQFMATKAHIKLNTKKLYQADGYAVKELLKITSVLYNAMKTKGMEGSEIVEEDVNKFKFDLGSKIADLKAARQLASEITSKGASLYDLLGMEVELREMRTEAIARPLEINETEKVMRIAIKEILTQVQKTKDLLNNVASDEANLEAKIEKRKLELERNRKRLETLQSVRPCFMDEYEKTEEELQKQYDTYLEKFQNLTYLEQQLEDHHRMEQERFEEAKNTLCLIQNKLKEEEKRLLKSGSNDDSDIDIQEDDESDSELEERRLPKPQTAMEMLMQGRPGKRIVGTMQGGDSDDNEDSEESEIDMEDDDDEDDDLEDESISLSPTKPNRRVWKSEPLDESDNDF

• Full Name: clusterin associated protein 1
• Calculated Molecular Weight: 413 aa, 48 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC017070
• Gene Symbol: CLUAP1
• Gene ID (NCBI): 23059
• RRID: AB_2083477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AJ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	WB,IF	Mol Cell Proteomics; CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.; Authors - Tina Beyer ; KO Validated
human	WB	Hum Mol Genet; Defective IFT57 underlies a novel cause of Bardet-Biedl syndrome; Authors - Alexandra Nitoiu
human	IF	Elife; A hierarchical pathway for assembly of the distal appendages that organize primary cilia; Authors - Tomoharu Kanie