CMBL Polyclonal antibody

CMBL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17409-1-AP

Synonyms

CMBL

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Validation Data Gallery

• 

  WB analysis using 17409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17409-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17409-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17409-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17409-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

17409-1-AP targets CMBL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CMBL fusion protein Ag11442
• Full Name: carboxymethylenebutenolidase homolog (Pseudomonas)
• Calculated Molecular Weight: 245 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001573
• Gene Symbol: CMBL
• Gene ID (NCBI): 134147
• RRID: AB_10596776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell Rep; p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP; Authors - Yingdan Huang