CMBL Polyclonal antibody

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• CMBL Polyclonal antibody (17409-1-AP)

CMBL Polyclonal Antibody for WB, ELISA

Cat No. 17409-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CMBL

• 

  WB analysis using 17409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 17409-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 17409-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 17409-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 17409-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17409-1-AP (CMBL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17409-1-AP targets CMBL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11442

  Product name: Recombinant human CMBL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-245 aa of BC001573

  Sequence: MANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHAQKIGIVGFCWGGTAVHHLMMKYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLNKYM

• Full Name: carboxymethylenebutenolidase homolog (Pseudomonas)
• Calculated Molecular Weight: 245 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001573
• Gene Symbol: CMBL
• Gene ID (NCBI): 134147
• RRID: AB_10596776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96DG6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CMBL Polyclonal Antibody (Cat# 17409-1-AP) has 1 citation. It was published in Cell Reports (2023) in a study investigating how p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP. The research field encompasses cancer metabolism and glycolysis regulation. The antibody was applied in Western Blot and IHC on human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Cell Rep; p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP; Authors - Yingdan Huang