CMIP Polyclonal antibody

• Featured Product
• KD/KO Validated

CMIP Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 12851-1-AP

Synonyms

TCMIP, Tc Mip, c-Mip, C-Maf-inducing protein, c Mip

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Validation Data Gallery

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  WB analysis of K-562 using 12851-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 12851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 12851-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 12851-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12851-1-AP (CMIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 12851-1-AP

  IP result of anti-CMIP (IP:12851-1-AP, 4ug; Detection:12851-1-AP 1:800) with Raji cells lysate 1800 ug.

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  IHC staining of human stomach using 12851-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12851-1-AP (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12851-1-AP targets CMIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CMIP fusion protein Ag3475
• Full Name: c-Maf-inducing protein
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC038113
• Gene Symbol: CMIP
• Gene ID (NCBI): 80790
• RRID: AB_2081366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.

Publications

Species	Application	Title
mouse	WB,IF	Cell Signal; Inhibition of nephrin activation by c-mip through Csk-Cbp-Fyn axis plays a critical role in Angiotensin II-induced podocyte damage.; Authors - Yu Lixia L ; KD Validated
mouse	WB	Cell Physiol Biochem; Induction of C-Mip by IL-17 Plays an Important Role in Adriamycin-Induced Podocyte Damage.; Authors - Yanbo Liu
human	WB,IHC	Biomed Res Int; CMIP Promotes Proliferation and Metastasis in Human Glioma.; Authors - Bin Wang ; KD Validated
human	WB,IHC	Pathol Res Pract; PMID: 31822364; Authors - Ru Xiang ; KD Validated
		Mol Med Rep; c‑Maf inducing protein inhibits cofilin‑1 activity and alters podocyte cytoskeleton organization.; Authors - Lixia Yu
human	WB,IHC	Mol Med Rep; CMIP is oncogenic in human gastric cancer cells.; Authors - Jianlin Zhang