CMPK1 Monoclonal antibody, PBS Only

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  WB analysis using 67519-1-Ig (same clone as 67519-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67519-1-Ig (CMPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67519-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67519-1-Ig (same clone as 67519-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CMPK1 antibody (67519-1-Ig, Clone: 2D11G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67519-1-PBS in a different storage buffer formulation.

CMPK1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67519-1-PBS

Clone No.2D11G7

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

CMK, CMPK, CMPK1, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP CMP kinase, UMP CMPK, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase

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Product Information

67519-1-PBS targets CMPK1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30084

  Product name: Recombinant human CMPK1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-228 aa of BC014961

  Sequence: MLSRCRSRLLHVLGLSFLLQTRRPILLCSPRLMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

• Full Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 22-26 kDa
• GenBank Accession Number: BC014961
• Gene Symbol: CMPK1
• Gene ID (NCBI): 51727
• RRID: AB_2882739
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30085
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.