CMPK2 Polyclonal antibody
CMPK2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25877-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
25877-1-AP targets CMPK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CMPK2 fusion protein Ag22885 |
Full Name | cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial |
Calculated Molecular Weight | 449 aa, 49 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC141802 |
Gene Symbol | CMPK2 |
Gene ID (NCBI) | 129607 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CMPK2 belongs to the thymidylate kinase family. CMPK2 may participate in dUTP and dCTP synthesis in mitochondria. It is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy of CMPK2 is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. CMPK2 may be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs.
Protocols
Product Specific Protocols | |
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WB protocol for CMPK2 antibody 25877-1-AP | Download protocol |
IHC protocol for CMPK2 antibody 25877-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |