CNBP Polyclonal antibody

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  WB analysis using 12103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12103-1-AP (CNBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 12103-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CNBP antibody (12103-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

CNBP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 12103-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12103-1-AP targets CNBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2747

  Product name: Recombinant human CNBP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-170 aa of BC014911

  Sequence: MSSNECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTIEATA

• Full Name: CCHC-type zinc finger, nucleic acid binding protein
• Calculated Molecular Weight: 170 aa, 19 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC014911
• Gene Symbol: CNBP
• Gene ID (NCBI): 7555
• RRID: AB_3085390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62633
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cellular nucleic acid binding protein (CNBP) has been implicated in vertebrate craniofacial development and in myotonic dystrophy type 2 (DM2) and sporadic inclusion body myositis (sIBM) human diseases [PMID:21277287]. CNBP is a small and strikingly conserved single-stranded nucleic acid binding protein that is able to bind DNA as well as RNA. This ssDNA- and RNA-binding protein with broad sequence specificity is participated in diverse cellular functions, including transcription, translation and through them cell proliferation and survival [PMID:20960530].