Tested Applications
| Positive WB detected in | Caco-2 cells, HeLa cells, mouse heart tissue, mouse testis tissue, U-251 cells |
| Positive IHC detected in | mouse spleen tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14717-1-AP targets CNBP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6441 Product name: Recombinant human CNBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC000288 Sequence: MSSNECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDACYNCGRGGHIAKDCKEPKREREQCCYNCGKPGHLARDCDHADEQKCYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSKTSEVNCYRCGESGHLARECTIEATA Predict reactive species |
| Full Name | CCHC-type zinc finger, nucleic acid binding protein |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 20-25 kDa |
| GenBank Accession Number | BC000288 |
| Gene Symbol | CNBP |
| Gene ID (NCBI) | 7555 |
| RRID | AB_2081548 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CNBP antibody 14717-1-AP | Download protocol |
| WB protocol for CNBP antibody 14717-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-CNBP antibody (Cat# 14717-1-AP) has 8 total citations published in journals including Cell Death & Disease, Journal of Translational Medicine, Journal of Medicinal Chemistry, PLoS Biology, Molecular & Cellular Proteomics, iScience, and Reproduction. Research fields span oncology (esophageal, gastric, colorectal, and hepatocellular carcinoma), rheumatoid arthritis, testicular development and spermatogenesis, and quantitative proteomics. Applications include WB, IF, and ELISA in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Dis USP5 regulates purine metabolism and represents a therapeutic target in esophageal cancer. | ||
Cell Death Dis A new candidate tumor suppressor tRF-Ser inhibits gastric cancer progression by regulating the CNBP/HSPA8 axis. | ||
J Transl Med Single-cell and spatial profiling of peripheral blood and synovium unveils pro-arthritis CNBP(+) myeloid cells. | ||
J Med Chem Discovery of Gut-Restricted PRMT5 Inhibitors to Intercept Colorectal Cancer in Patients with Genetic Loss of Tumor Suppressor Adenomatous Polyposis Coli. | ||
PLoS Biol Phosphoglucomutase 1 inhibits hepatocellular carcinoma progression by regulating glucose trafficking.
| ||
Mol Cell Proteomics Quantitative proteomics reveals the essential roles of STIM1 in the testicular cord formation in mouse testis. |
