CNBP Monoclonal antibody

• Featured Product
• KD/KO Validated

CNBP Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1H1D12

Reviews (1)

Cat no : 67109-1-Ig

Synonyms

CNBP, CNBP1, DM2, FLJ11631, PROMM, RNF163, ZCCHC22, Zinc finger protein 9, ZNF9

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Validation Data Gallery

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  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67109-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67109-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 67109-1-Ig

  IP result of anti-CNBP (IP:67109-1-Ig, 5ug; Detection:67109-1-Ig 1:500) with HeLa cells lysate 1600 ug.

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, A549 cells, NCI-H1299 cells, Caco-2 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HepG2 cells, K-562 cells, PC-12 cells, Neuro-2a cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

67109-1-Ig targets CNBP in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNBP fusion protein Ag7114
• Full Name: CCHC-type zinc finger, nucleic acid binding protein
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19-25 kDa
• GenBank Accession Number: BC000288
• Gene Symbol: CNBP
• Gene ID (NCBI): 7555
• RRID: AB_2882413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP,WB	Nat Microbiol; The SARS-CoV-2 RNA-protein interactome in infected human cells.; Authors - Nora Schmidt ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katherine (Verified Customer) (09-25-2023)

The band was clear

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T