CNDP1 Polyclonal antibody

CNDP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17759-1-AP

Synonyms

CPGL2, CNDP dipeptidase 1, CN1, Carnosine dipeptidase 1, Beta-Ala-His dipeptidase

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Validation Data Gallery

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  WB analysis of HEK-293 using 17759-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17759-1-AP (CNDP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17759-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17759-1-AP (CNDP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 17759-1-AP

  Immunofluorescent analysis of A431 cells using 17759-1-AP (CNDP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells
• Positive IHC detected in: human prostate cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

17759-1-AP targets CNDP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNDP1 fusion protein Ag12127
• Full Name: carnosine dipeptidase 1 (metallopeptidase M20 family)
• Calculated Molecular Weight: 508 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC110295
• Gene Symbol: CNDP1
• Gene ID (NCBI): 84735
• RRID: AB_2878435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-Ala-His dipeptidase (CNDP1) encodes the secreted serum carnosinase, which is synthesized in the brain and secreted into the systematic circulation, CNDP1 hydrolyzes L-carnosine and is essential for glucose metabolism and the transportation of activated fatty acids across the mitochondrial membrane (PMID: 32111749, 16046297).

Publications

Species	Application	Title
human	IHC	Amino Acids; Serum and urinary carnosinase-1 correlate with kidney function and inflammation; Authors - Shiqi Zhang