Tested Applications
| Positive WB detected in | HepG2 cells, LNCaP cells, mouse kidney tissue, rat kidney rat kidney |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14925-1-AP targets CNDP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6684 Product name: Recombinant human CNDP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 124-475 aa of BC001375 Sequence: KLYGRGSTDDKGPVAGWINALEAYQKTGQEIPVNVRFCLEGMEESGSEGLDELIFARKDTFFKDVDYVCISDNYWLGKKKPCITYGLRGICYFFIEVECSNKDLHSGVYGGSVHEAMTDLILLMGSLVDKRGNILIPGINEAVAAVTEEEHKLYDDIDFDIEEFAKDVGAQILLHSHKKDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPRKVVGKFSIRLVPNMTPEVVGEQVTSYLTKKFAELRSPNEFKVYMGHGGKPWVSDFSHPHYLAGRRAMKTVFGVEPDLTREGGSIPVTLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRYNYIEGTKMLAAYLYEVSQLKD Predict reactive species |
| Full Name | CNDP dipeptidase 2 (metallopeptidase M20 family) |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 44-53 kDa |
| GenBank Accession Number | BC001375 |
| Gene Symbol | CNDP2 |
| Gene ID (NCBI) | 55748 |
| RRID | AB_2081682 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KP4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa.
The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CNDP2 antibody 14925-1-AP | Download protocol |
| IHC protocol for CNDP2 antibody 14925-1-AP | Download protocol |
| IP protocol for CNDP2 antibody 14925-1-AP | Download protocol |
| WB protocol for CNDP2 antibody 14925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CNDP2 Polyclonal antibody (Cat# 14925-1-AP) has 20 citations spanning high-impact journals including Nature, Cell, Nature Metabolism, Cell Death & Discovery, Acta Neuropathologica, Molecular Medicine, Glia, and BMC Cancer. Research fields covered include cancer biology (gastric, colon, prostate, renal), amino acid and ketone metabolism, obesity, neurodegeneration (Alzheimer's, SMA), inflammation, oxidative stress, and aging. Applications cited are predominantly WB and IHC.
| Species | Application | Title |
|---|---|---|
Nat Metab Lac-Phe mediates the effects of metformin on food intake and body weight
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Cell Death Discov Exercise-induced lactate suppresses ccRCC via CNDP2-mediated depletion of intracellular amino acids | ||
Acta Neuropathol The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease. | ||
Cell Chem Biol Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics.
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