CNDP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CNDP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 14925-1-AP

Synonyms

HEL-S-13, Glutamate carboxypeptidase-like protein 1, EC:3.4.13.18, Cytosolic non-specific dipeptidase, CPGL

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Validation Data Gallery

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  WB analysis using 14925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14925-1-AP (CNDP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14925-1-AP

  IP result of anti-CNDP2 (IP:14925-1-AP, 4ug; Detection:14925-1-AP 1:1000) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human testis using 14925-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14925-1-AP (CNDP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14925-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14925-1-AP (CNDP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 14925-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNDP2 antibody (14925-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HepG2 cells, LNCaP cells, mouse kidney tissue, rat kidney rat kidney
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

14925-1-AP targets CNDP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNDP2 fusion protein Ag6684
• Full Name: CNDP dipeptidase 2 (metallopeptidase M20 family)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 44-53 kDa
• GenBank Accession Number: BC001375
• Gene Symbol: CNDP2
• Gene ID (NCBI): 55748
• RRID: AB_2081682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa. The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).

Publications

Species	Application	Title
	IHC	Acta Neuropathol; The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease.; Authors - David C Hondius
mouse	WB	Cell Chem Biol; Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics.; Authors - Joon T Kim ; KO Validated
human	WB,IHC	Mol Med; Underexpressed CNDP2 participates in gastric cancer growth inhibition through activating the MAPK signaling pathway.; Authors - Zhenwei Zhang
human	WB,IF	BMC Cancer; The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study; Authors - Ying E
human	WB	PLoS One; Quantitative proteomics approach to screening of potential diagnostic and therapeutic targets for laryngeal carcinoma.; Authors - Li Li
human	WB,IHC	BMC Gastroenterol; Up-regulation of CNDP2 facilitates the proliferation of colon cancer.; Authors - Conglong Xue ; KD Validated