CNKSR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CNKSR1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10885-1-AP

Synonyms

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

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Validation Data Gallery

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  WB analysis of HT-29 using 10885-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10885-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-29 using 10885-1-AP

  IP result of anti-CNKSR1 (IP:10885-1-AP, 4ug; Detection:10885-1-AP 1:1000) with HT-29 cells lysate 4000 ug.

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  IHC staining of human pancreas cancer using 10885-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10885-1-AP (CNKSR1 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10885-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10885-1-AP (CNKSR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells, human brain tissue
• Positive IP detected in: HT-29 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

10885-1-AP targets CNKSR1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNKSR1 fusion protein Ag1333
• Full Name: connector enhancer of kinase suppressor of Ras 1
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC011604
• Gene Symbol: CNKSR1
• Gene ID (NCBI): 10256
• RRID: AB_2276387
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.

Publications

Species	Application	Title
human	WB,IHC	Life Sci Alliance; CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP.; Authors - Kanako Nishiyama ; KD Validated
human	IHC	Cancer Rep (Hoboken); Identification of hub genes in bladder cancer based on weighted gene co-expression network analysis from TCGA database.; Authors - Lei Wang
human	IHC	Heliyon; Identification of CNKSR1 as a biomarker for "cold" tumor microenvironment in lung adenocarcinoma: An integrative analysis based on a novel workflow; Authors - Qidong Cai