CNKSR1 Monoclonal antibody, PBS Only

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  WB analysis of MCF-7 using 66796-1-Ig (same clone as 66796-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66796-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66796-1-Ig (same clone as 66796-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66796-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 66796-1-Ig (same clone as 66796-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66796-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Caco-2 using 66796-1-Ig (same clone as 66796-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66796-1-PBS in a different storage buffer formulation.

CNKSR1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66796-1-PBS

Clone No.1E11H8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

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Product Information

66796-1-PBS targets CNKSR1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27855

  Product name: Recombinant human CNKSR1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 308-720 aa of BC011604

  Sequence: LSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAILPAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFMGPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPFIFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSPAQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQEQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQNRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

• Full Name: connector enhancer of kinase suppressor of Ras 1
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC011604
• Gene Symbol: CNKSR1
• Gene ID (NCBI): 10256
• RRID: AB_2882140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q969H4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.