CNKSR2 Polyclonal antibody, PBS Only

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• CNKSR2 Polyclonal antibody, PBS Only (24054-1-PBS)

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  WB analysis using 24054-1-AP (same clone as 24054-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24054-1-AP (CNKSR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24054-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 24054-1-AP (same clone as 24054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 24054-1-AP (CNKSR2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24054-1-PBS in a different storage buffer formulation.

CNKSR2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 24054-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CNK homolog protein 2, CNK2, Connector enhancer of KSR 2, KIAA0902, KSR2

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Product Information

24054-1-PBS targets CNKSR2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21183

  Product name: Recombinant human CNKSR2 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 400-558 aa of BC126121

  Sequence: RKRFNIVEEDTVLYCYEYEKGRSSSQGRRESTPTYGKLRPISMPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKKGDKSNSPTHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAGK

• Full Name: connector enhancer of kinase suppressor of Ras 2
• Calculated Molecular Weight: 1034 aa, 118 kDa
• Observed Molecular Weight: 110 kDa, 130 kDa
• GenBank Accession Number: BC126121
• Gene Symbol: CNKSR2
• Gene ID (NCBI): 22866
• RRID: AB_3669430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8WXI2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The Connector Enhancer of Kinase Suppressor of Ras-2 (CNKSR2), also known as CNK2 or MAGUIN, KIAA0902 and KSR2, is a scaffolding molecule that contains functional protein binding domains (PMID: 35053419). CNKSR2 interacts with different molecules, including RAF1, ARHGAP39, and CYTH2, and regulates the Mitogen-Activated Protein Kinase (MAPK) cascade and small GTPase signaling (PMID: 35053419). The study identify a molecular complex comprising CNKSR2 and the guanine nucleotide exchange factor (GEF) for ARF small GTPases, CYTH2, that is necessary for the proper development of granule neurons in the mouse hippocampus (PMID: 34800437). CNKSR2 plays a key role in regulating cell proliferation, and differentiation through multiple receptor tyrosine kinase pathways (PMID: 35053419). The molecular weight of CNKSR2 is mainly 117 kDa (PMID: 29534682). It has also been reported that CNKSR2 immunoblotted signal of about 110 kDa was detected, while a 130 kDa signal was detected (PMID: 34800437).