CNKSR3 Polyclonal antibody

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  WB analysis of human brain using 14084-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14084-1-AP (CNKSR3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14084-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14084-1-AP (CNKSR3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14084-1-AP

  IP result of anti-CNKSR3 (IP:14084-1-AP, 4ug; Detection:14084-1-AP 1:500) with mouse brain tissue lysate 3440ug.

CNKSR3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14084-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

CNK homolog protein 3, CNK3, CNKSR family member 3, CNKSR3, Connector enhancer of KSR 3, MAGI1, Maguin like protein

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14084-1-AP targets CNKSR3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5213

  Product name: Recombinant human CNKSR3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-352 aa of BC060761

  Sequence: MEPVTKWSPKQVVDWTRGLDDCLQQYVHKFEREKINGEQLLQISHQDLEELGVTRIGHQELVLEAVDLLCALNYGLETDNMKNLVLKLRASSHNLQNYISSRRKSPAYDGNTSRKAPNEFLTSVVELIGAAKALLAWLDRAPFTGITDFSVTKNKIIQLCLDLTTTVQKDCFVAEMEDKVLTVVKVLNGICDKTIRSTTDPVMSQCACLEEVHLPNIKPGEGLGMYIKSTYDGLHVITGTTENSPADRSQKIHAGDEVIQVNQQTVVGWQLKNLVKKLRENPTGVVLLLKKRPTGSFNFTPAPLKNLRWKPPLVQTSPPPATTQSPESTMDTSLKKEKSAILDLYIPPPPAV

• Full Name: CNKSR family member 3
• Calculated Molecular Weight: 555 aa, 62 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: BC060761
• Gene Symbol: CNKSR3
• Gene ID (NCBI): 154043
• RRID: AB_2229651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P9H4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connector enhancer of kinase suppressor of ras 3 (CNKSR3) gene encodes CNK homolog protein 3 (CNK3). CNK proteins, originally identified in Drosophila, were described to regulate the RAS/ERK pathway at the level of the RAS protein kinase. CNKSR3, homologue of scaffold proteins involved in MAPK pathway regulation, is a direct target of mineralocorticoid receptor (MR). CNK3 coordinates the assembly of a multiprotein epithelial sodium channel (ENaC) -regulatory complex, and hence plays a central role in sodium homeostasis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human, mouse	WB	J Biol Chem; Scaffold protein connector enhancer of kinase suppressor of Ras isoform 3 (CNK3) coordinates assembly of a multiprotein epithelial sodium channel (ENaC)-regulatory complex.; Authors - Soundararajan Rama R