CNNM3 Polyclonal antibody

CNNM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13976-1-AP

Synonyms

Cyclin-M3, cyclin M3, Ancient conserved domain-containing protein 3, ACDP3

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Validation Data Gallery

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  WB analysis using 13976-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13976-1-AP (CNNM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13976-1-AP

  IP result of anti-CNNM3 (IP:13976-1-AP, 4ug; Detection:13976-1-AP 1:500) with K-562 cells lysate 3600ug.

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  IHC staining of human spleen using 13976-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13976-1-AP (CNNM3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 13976-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13976-1-AP (CNNM3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 13976-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CNNM3 antibody (13976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, K-562 cells, Raji cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

13976-1-AP targets CNNM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNNM3 fusion protein Ag5012
• Full Name: cyclin M3
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC037272
• Gene Symbol: CNNM3
• Gene ID (NCBI): 26505
• RRID: AB_2082153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human Cyclin M 3 (CNNM3) has five transmembrane domains located at the N-terminal region and a C terminus containing a CBS pair domain and a cyclin box, both of which are part of the ACD region. CNNM3 magnesium transporter raises the intracellular magnesium content of cancer cells contributing to increased tumor growth and the acquisition of oncogenic properties (PMID: 24632616).

Publications

Species	Application	Title
human	WB,IP	Oncogene; The protein tyrosine phosphatase PRL-2 interacts with the magnesium transporter CNNM3 to promote oncogenesis.; Authors - S Hardy
mouse	WB,IF	JCI Insight; PRL2 links magnesium flux and sex-dependent circadian metabolic rhythms.; Authors - Noriko Uetani
human	WB,IP	Proc Natl Acad Sci U S A; PRL-1/2 phosphatases control TRPM7 magnesium-dependent function to regulate cellular bioenergetics; Authors - Serge Hardy