CNNM4 Polyclonal antibody, PBS Only

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• CNNM4 Polyclonal antibody, PBS Only (14066-1-PBS)

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  WB analysis of HEK-293 using 14066-1-AP (same clone as 14066-1-PBS)

  WB result of CNNM4 antibody (14066-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNNM4 transfected HEK-293 cells. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

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  WB analysis using 14066-1-AP (same clone as 14066-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14066-1-AP (CNNM4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 14066-1-AP (same clone as 14066-1-PBS)

  IP result of anti-CNNM4 (IP:14066-1-AP, 4ug; Detection:14066-1-AP 1:1000) with mouse testis tissue lysate 4400ug. This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14066-1-AP (same clone as 14066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14066-1-AP (CNNM4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14066-1-AP (same clone as 14066-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (14066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14066-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CNNM4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14066-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ACDP4, Ancient conserved domain-containing protein 4, cyclin M4, Cyclin-M4, KIAA1592

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Product Information

14066-1-PBS targets CNNM4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5121

  Product name: Recombinant human CNNM4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 420-775 aa of BC063295

  Sequence: DILYVKDLAFVDPDDCTPLKTITRFYNHPVHFVFHDTKLDAMLEEFKKGKSHLAIVQKVNNEGEGDPFYEVLGLVTLEDVIEEIIKSEILDESDMYTDNRSRKRVSEKNKRDFSAFKDADNELKVKISPQLLLAAHRFLATEVSQFSPSLISEKILLRLLKYPDVIQELKFDEHNKYYARHYLYTRNKPADYFILILQGKVEVEAGKENMKFETGAFSYYGTMALTSVPSDRSPAHPTPLSRSASLSYPDRTDVSTAATLAGSSNQFGSSVLGQYISDFSVRALVDLQYIKITRQQYQNGLLASRMENSPQFPIDGCTTHMENLAEKSELPVVDETTTLLNERNSLLHKASHENAI

• Full Name: cyclin M4
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC063295
• Gene Symbol: CNNM4
• Gene ID (NCBI): 26504
• RRID: AB_2878009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P4Q7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).