CNOT10 Polyclonal antibody

CNOT10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 15938-1-AP

Synonyms

CNOT10

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Validation Data Gallery

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  WB analysis using 15938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15938-1-AP (CNOT10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15938-1-AP

  IP result of anti-CNOT10 (IP:15938-1-AP, 4ug; Detection:15938-1-AP 1:300) with mouse brain tissue lysate 5200ug.

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  IHC staining of human colon cancer using 15938-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15938-1-AP (CNOT10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNOT10 antibody (15938-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 15938-1-AP

  Immunofluorescent analysis of HepG2 cells, using CNOT10 antibody 15938-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

15938-1-AP targets CNOT10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNOT10 fusion protein Ag7654
• Full Name: CCR4-NOT transcription complex, subunit 10
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC002928
• Gene Symbol: CNOT10
• Gene ID (NCBI): 25904
• RRID: AB_2229678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNOT10, also named as CCR4-NOT transcription complex subunit 10, is a 744 amino acid protein, which belongs to the CNOT10 family. CNOT10 lacalizes in the cytoplasm and nucleus. CNOT10 is a component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.

Publications

Species	Application	Title
	WB	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	Nat Commun; RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.; Authors - Fabian Poetz
human	WB	Nucleic Acids Res; Alternative splicing of CNOT7 diversifies CCR4-NOT functions.; Authors - Clément Chapat
human	WB	Cell Rep; The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions; Authors - Fabienne Mauxion
human	WB	Nucleic Acids Res; Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAs.; Authors - F?rber Valentin V
		RNA Biol; C2ORF29/CNOT11 and CNOT10 form a new module of the CCR4-NOT complex.; Authors - Mauxion Fabienne F

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

Working very well to detect CNOT10 by WB in HeLa cells (1/1000 dilution - Overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa