Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, mouse cerebellum tissue, rat kidney tissue, rat ovary tissue, mouse kidney tissue, mouse ovary tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15938-1-AP targets CNOT10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7654 Product name: Recombinant human CNOT10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 568-717 aa of BC002928 Sequence: NPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRAPQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLALQIIKRNQLLPAVKTHSEVRKKPVFQPVHPIQPIQMSAFTTVQRK Predict reactive species |
| Full Name | CCR4-NOT transcription complex, subunit 10 |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 82 kDa |
| GenBank Accession Number | BC002928 |
| Gene Symbol | CNOT10 |
| Gene ID (NCBI) | 25904 |
| RRID | AB_2229678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9A5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CNOT10, also named as CCR4-NOT transcription complex subunit 10, is a 744 amino acid protein, which belongs to the CNOT10 family. CNOT10 lacalizes in the cytoplasm and nucleus. CNOT10 is a component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CNOT10 antibody 15938-1-AP | Download protocol |
| IHC protocol for CNOT10 antibody 15938-1-AP | Download protocol |
| IP protocol for CNOT10 antibody 15938-1-AP | Download protocol |
| WB protocol for CNOT10 antibody 15938-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CNOT10 Polyclonal antibody (Cat# 15938-1-AP) has 8 citations published in Nature Cancer, Nature Communications, Molecular Cell, Nucleic Acids Research, Genome Biology, Cell Reports, and RNA Biology. Research fields include mRNA deadenylation and degradation, CCR4-NOT complex structure and function, alternative splicing, ferroptosis regulation, gene expression control, and viral inflammasome evasion. All cited applications are Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Nat Cancer ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1. | ||
Nat Commun RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation. | ||
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
Nucleic Acids Res Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAs. | ||
Genome Biol Control of immediate early gene expression by CPEB4-repressor complex-mediated mRNA degradation |
Reviews
What customers say
The single review reports successful use of this antibody for Western Blot to detect CNOT10 in HeLa cells. The user applied a 1/1000 dilution with overnight incubation at 4°C and rated the product 5 stars, describing it as working very well. Overall, the feedback is positive, confirming reliable performance for CNOT10 detection by WB under the stated conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-13-2024) | Working very well to detect CNOT10 by WB in HeLa cells (1/1000 dilution - Overnight incubation at 4°C)
|
