Tested Applications
| Positive WB detected in | NIH/3T3 cells, HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10313-1-AP targets CNOT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0347 Product name: Recombinant human CNOT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-540 aa of BC002597 Sequence: QVLPDGRVTNIPQGMVTDQFGMIGLLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTYYFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF Predict reactive species |
| Full Name | CCR4-NOT transcription complex, subunit 2 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC002597 |
| Gene Symbol | CNOT2 |
| Gene ID (NCBI) | 4848 |
| RRID | AB_2245072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZN8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Ccr4-Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CNOT2 antibody 10313-1-AP | Download protocol |
| WB protocol for CNOT2 antibody 10313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CNOT3 antibody (Cat# 10313-1-AP) has 9 citations published in journals including Cell Mol Life Sci, Cells, Stem Cell Reports, Stem Cells, RNA Biology, Stem Cells Dev, bioRxiv, and Methods Mol Biol. Research fields span mRNA deadenylation and regulation, Ccr4-Not complex function, pluripotency and stem cell maintenance, germ cell development, and transcription-mediated genome instability, with applications primarily in Western blot and immunofluorescence across human, mouse, and pig species.
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs | ||
Cells Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability | ||
Stem Cells Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.
| ||
RNA Biol Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability. | ||
Stem Cells Dev Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
|
Reviews
What customers say
All four reviews rate the antibody 5 stars. Users report clear, clean signals in Western Blot (tested in HeLa and HEK293T cells at 1:2000) and reliable performance in Immunofluorescence (RPE-1 and HEK293T cells at 1:100 overnight at 4°C). One reviewer also plans to validate the antibody for immunoprecipitation. Overall, customers are highly satisfied with the antibody's specificity and ease of use across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Maria (Verified Customer) (10-03-2025) | I used the antibody for western blot detection in HeLa cells and the signal is very clear.
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Aditya (Verified Customer) (10-08-2024) | very clean blot
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Abe (Verified Customer) (04-25-2024) | Worked well for IF at 1:100 overnight at 4c in RPE-1 cells.
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Ayse (Verified Customer) (06-08-2022) | Used for IF experiment (dilution 1/100) and WB experiment (1/2000). The antibody works well for the 2 kind of experiment. In few weeks, I will also test for IP.
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