CNOT3 Polyclonal antibody

• Featured Product
• KD/KO Validated

CNOT3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (2)

Cat no : 11135-1-AP

Synonyms

NOT3, LENG2, KIAA0691, CCR4-NOT transcription complex subunit 3, CCR4-associated factor 3

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Validation Data Gallery

• 

  WB analysis of Raji using 11135-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 11135-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11135-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11135-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11135-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11135-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11135-1-AP

  IP result of anti-CNOT3 (IP:11135-1-AP, 4ug; Detection:11135-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IF Staining of HeLa using 11135-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CNOT3 antibody (11135-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, HEK-293 cells, HT-1080 cells, human brain tissue, K-562 cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

11135-1-AP targets CNOT3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CNOT3 fusion protein Ag1587
• Full Name: CCR4-NOT transcription complex, subunit 3
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC016474
• Gene Symbol: CNOT3
• Gene ID (NCBI): 4849
• RRID: AB_2229682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].

Publications

Species	Application	Title
mouse	IF	Cell; A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.; Authors - G Gregory Neely ; KO Validated
human	WB	Nucleic Acids Res; Alternative splicing of CNOT7 diversifies CCR4-NOT functions.; Authors - Clément Chapat
human	WB	RNA; An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.; Authors - Ewan K S McRae
human	WB	Apoptosis; PMID: 31177396; Authors - Lin Jing ; KD Validated
human	WB	Nat Cancer; ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.; Authors - Tong Zhang
mouse	WB,IF	bioRxiv; Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance; Authors - Qing Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

Great CNOT3 bands by WB in HeLa cells (1/1000 dilution, overnight incubation at 4°C).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Hannah (Verified Customer) (02-06-2019)

Works well and specifically for WB on human fibroblast lysates.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/5000 overnight
• Cell Tissue Type: human fibroblast (NHDF)

CNOT3 Antibody Western Blot, validation (1/5000 overnight dilution) in human fibroblast (NHDF) (Cat no:11135-1-AP)