CNOT3 Polyclonal antibody

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CNOT3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 11135-1-AP Copied!

Publications (17)

Reviews (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

NOT3, LENG2, KIAA0691, CCR4-NOT transcription complex subunit 3, CCR4-associated factor 3

• 

  WB analysis of Raji using 11135-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HT-1080 using 11135-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 11135-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 11135-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11135-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 11135-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 11135-1-AP

  IP result of anti-CNOT3 (IP:11135-1-AP, 4ug; Detection:11135-1-AP 1:1000) with HeLa cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11135-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CNOT3 antibody (11135-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Raji cells, HEK-293 cells, HT-1080 cells, human brain tissue, K-562 cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11135-1-AP targets CNOT3 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1587

  Product name: Recombinant human CNOT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 449-753 aa of BC016474

  Sequence: NASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNNSGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ

• Full Name: CCR4-NOT transcription complex, subunit 3
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC016474
• Gene Symbol: CNOT3
• Gene ID (NCBI): 4849
• RRID: AB_2229682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75175
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].

Publications

What published studies show

The anti-CNOT3 antibody (Cat# 11135-1-AP) has 17 total citations. These appear in journals including Science, Cell, Nature Cancer, Nature Communications, Nucleic Acids Research, Cell Death & Discovery, Apoptosis, Stem Cell Research & Therapy, Cell Reports, Cells, EMBO Reports, RNA, Biotechnology Journal, Food and Chemical Toxicology, and bioRxiv. Research fields span mRNA decay and stability, the CCR4-NOT complex, RNA-binding proteins, cancer (lung, ovarian, prostate), ferroptosis, and germ cell development, with applications mainly in Western blot, IP, and IF.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Science; Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes; Authors - Xiaoqiang Zhu
human	WB,IP	Science; Human DHX29 detects nonoptimal codon usage to regulate mRNA stability.; Authors - Fabian Hia
mouse	IF	Cell; A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.; Authors - G Gregory Neely ; KO Validated
human	WB	Nat Cancer; ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.; Authors - Tong Zhang
human	WB	Nat Commun; Regulation by the RNA-binding protein Unkempt at its effector interface; Authors - Kriti Shah
human	WB	Nucleic Acids Res; Alternative splicing of CNOT7 diversifies CCR4-NOT functions.; Authors - Clément Chapat

Reviews

5.0 out of 5

3 verified customer reviews

What customers say

All three reviews are 5-star ratings. Users report the antibody performs well for Western blotting in HeLa cells (1/1000, overnight at 4°C) and human fibroblast lysates (1/5000, overnight), producing clear and specific CNOT3 bands. It also works effectively for immunoprecipitation at 4 µg/IP against 600 µg of protein lysate. Overall, customers find it reliable and specific for both WB and IP applications.

Automatically generated from the 3 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Maria (Verified Customer) (10-09-2025)

It works perfectly for detection in WB and for IP at 4ug/IP for 600 ug of protein lysate.

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: HeLa

Roy (Verified Customer) (06-13-2024)

Great CNOT3 bands by WB in HeLa cells (1/1000 dilution, overnight incubation at 4°C).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

Hannah (Verified Customer) (02-06-2019)

Works well and specifically for WB on human fibroblast lysates.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/5000 overnight
• Cell Tissue Type: human fibroblast (NHDF)

CNOT3 Antibody Western Blot, validation (1/5000 overnight dilution) in human fibroblast (NHDF) (Cat no:11135-1-AP)