CNOT3 Monoclonal antibody

CNOT3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig , mouse, rat , rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E12C6

Cat no : 68436-1-Ig

Synonyms

CCR4 associated factor 3, CNOT3, KIAA0691, LENG2, NOT3, NOT3H

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Validation Data Gallery

• 

  WB analysis using 68436-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68436-1-Ig (CNOT3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68436-1-Ig targets CNOT3 in WB, ELISA applications and shows reactivity with Human, pig , mouse, rat , rabbit samples.

• Tested Reactivity: Human, pig , mouse, rat , rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNOT3 fusion protein Ag33646
• Full Name: CCR4-NOT transcription complex, subunit 3
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC016474
• Gene Symbol: CNOT3
• Gene ID (NCBI): 4849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].