Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68436-1-Ig targets CNOT3 in WB, ELISA applications and shows reactivity with Human, pig , mouse, rat , rabbit samples.
| Tested Reactivity | Human, pig , mouse, rat , rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33646 Product name: Recombinant human CNOT3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 449-753 aa of BC016474 Sequence: NASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNNSGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ Predict reactive species |
| Full Name | CCR4-NOT transcription complex, subunit 3 |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC016474 |
| Gene Symbol | CNOT3 |
| Gene ID (NCBI) | 4849 |
| RRID | AB_3085149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75175 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT3 antibody 68436-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The reviewer used it for Western Blot on HEK293 cells at a 1:1000 dilution and described it as an excellent antibody, noting that it performs significantly better with HRP detection than with fluorescence.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aditya (Verified Customer) (06-20-2024) | Excellent antibody, works significantly better with HRP than with flourescence
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