Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293T cells, HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12564-1-AP targets CNOT4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3163 Product name: Recombinant human CNOT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 273-572 aa of BC035590 Sequence: IDKPSDSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDIFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVAGEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA Predict reactive species |
| Full Name | CCR4-NOT transcription complex, subunit 4 |
| Calculated Molecular Weight | 575 aa, 64 kDa |
| Observed Molecular Weight | 64-85 kDa |
| GenBank Accession Number | BC035590 |
| Gene Symbol | CNOT4 |
| Gene ID (NCBI) | 4850 |
| RRID | AB_2082457 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95628 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CNOT4 antibody 12564-1-AP | Download protocol |
| WB protocol for CNOT4 antibody 12564-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CNOT4 Polyclonal antibody (Cat# 12564-1-AP) has 5 citations published in Science, Nature Communications, Cells, Journal of Cell Science, and Molecular Carcinogenesis. Research fields span mRNA decay via the CCR4-NOT complex, ribosomal quality control, transcription-mediated genome instability, nonsense-mediated mRNA decay, and bladder cancer progression involving autophagy regulation.
| Species | Application | Title |
|---|---|---|
Science Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes | ||
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Cells Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability
| ||
Mol Carcinog CNOT4 Suppresses Bladder Cancer Progression by Promoting Ubiquitination-Mediated Degradation of EIF2AK2 and Inhibiting Autophagy. |
Reviews
What customers say
Both reviewers rated this antibody 5 out of 5 stars for Western Blot. Users report it reliably detects CNOT4, with one noting clear visualization of all three CNOT4 bands. The recommended dilution is 1/1000 with overnight incubation at 4°C, and it performed well in HeLa cells. Overall, reviewers found the antibody to work perfectly out of the box with no issues mentioned.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Maria (Verified Customer) (11-09-2025) | It works perfectly detecting the three bands of CNOT4.
|
Roy (Verified Customer) (06-13-2024) | Works well to detect CNOT4 by WB (1/1000 dilution, incubation overnight at 4°C)
|
