Filter: AllWBIHCIF-PIF/ICC

WB analysis using 67798-1-Ig (same clone as 67798-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67798-1-Ig (CNOT4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67798-1-Ig (CNOT4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 67798-1-Ig (same clone as 67798-1-PBS)
WB result of CNOT4 antibody (67798-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT4 transfected MCF-7 cells. This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.
WB analysis of U2OS using 67798-1-Ig (same clone as 67798-1-PBS)
U2OS cells were subjected to SDS PAGE followed by western blot with 67798-1-Ig (CNOT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67798-1-Ig (same clone as 67798-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67798-1-Ig (CNOT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67798-1-Ig (same clone as 67798-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67798-1-Ig (CNOT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 67798-1-Ig (same clone as 67798-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 67798-1-Ig (same clone as 67798-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67798-1-Ig (same clone as 67798-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67798-1-PBS in a different storage buffer formulation.

CNOT4 Monoclonal antibody, PBS Only

CNOT4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 67798-1-PBS

Clone No.4H5H3

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67798-1-PBS targets CNOT4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag29964 Product name: Recombinant human CNOT4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 273-572 aa of BC035590 Sequence: IDKPSDSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDIFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVAGEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA Predict reactive species
Full Name	CCR4-NOT transcription complex, subunit 4
Calculated Molecular Weight	575 aa, 64 kDa
Observed Molecular Weight	64-85 kDa
GenBank Accession Number	BC035590
Gene Symbol	CNOT4
Gene ID (NCBI)	4850
RRID	AB_2918562
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O95628
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.